Published: 2019-04-30

Clinicobacteriological profile, antibiotic sensitivity patterns and mortality of neonatal sepsis in a tertiary care hospital in Kashmir, Jammu and Kashmir, India

Gh Rasool Wani, Nazir Ahmed, Mohd Irshad, Mohd Ashraf, Bashir Ahmed Teli


Background: Neonatal sepsis refers to generalized bacterial blood stream infection in first 28 days of life documented by positive blood cultures. It is one of leading causes of neonatal mortality. Objectives was to study clinicobacteriological, antibiotic sensitivity patterns and mortality of neonatal sepsis.

Methods: This prospective study was conducted in the Department of Pediatrics of Government Medical College Srinagar in collaboration with Department of Microbiology of same medical college after ethical clearance from ethical committee of Government Medical College Srinagar. One hundred (100) neonates out of 731 neonates admitted between octomber2007 and September 2008 with signs and symptoms of neonatal sepsis were included in our study by random sampling method. After history, examination and laboratory investigation blood culture results were analyzed by standard statistical methods.

Results: The blood culture was positive in 40% of neonates. Fifty one (51) neonates were males while as 49 were females. Sixty three (63) neonates had late onset of sepsis while as 37 had early onset sepsis. The positive  blood culture was more common in males, late onset sepsis, babies born in rural areas, home born, vaginal births, preterm and other  low birth weight neonates .The gram negative isolates were most common followed by positive ones .The best sensitivity of gram negative isolates was to ciprofloxacin followed by amikacin and cephalosporins while as gram positive isolates were sensitive to imipenum followed by vancomycin. Pseudomonas was most responsive to pipercillin +tazobactum combination. The neonatal mortality was 35% being higher in early onset sepsis and low birth weights.

Conclusions: This study depicts a high rate of neonatal sepsis, mainly caused by gram negative organisms followed by gram positive organisms with rising drug resistance that could bear far reaching implications to the times to come, mandating the implementation of sepsis preventive measures and administration of specific antibiotics.


Clinicobacteriological profile, Antibiotic sensitivity patterns, Mortality of neonatal sepsis

Full Text:



Trotman H, Bell Y, Thame M, Nicholson AM, Barton M. Predictors of poor outcome in neonatal sepsis admitted to university hospital of the West Indies. West Indian Med J. 2006 Mar;55(2):80-4.

Sathyamurthi B, Leela KV, Narayanababu R, Padmanaban S, Sreedevi S, Sujatha et al. Clinical and bacteriological profile of neonatal sepsis in a tertiary care hospital. Int J Sci Stud. 2016;4(8):57-60.

Rajana R, Bagri DR, Sharma JN, Agarwal V. Clinical and bacteriological profile of neonatal sepsis with emerging resistance patterns. Int J Contemp Pediatr. 2018;5:2203-8.

Vernago S, Sharland M, Kazembe P, Mwansambo C, Heath P. Neonatal sepsis: an international perspective. Arch Dis Child Fetal Neonatal Ed. 2005 May;90(3):F220-4.

Tallur SS, Kasturi AV, Nadgir SD, Krishna BV. Clinicobacteriological study of neonatal septicemia in Hubli. Indian J Pediatr. 2000 Mar;67(3):169-74.

Oommen SA, Saini S, Rahul RK. Bacteriological profile of Neonatal septicemia: A retrospective analysis from a tertiary care hospital in Loni. Int J Meds Res Health Sci. 2015;4(3):652-8.

Jyothi P, Basavaraj MC, Basavaraj PV. Bacteriological profile of neonatal septicemia and antibiotic susceptibility patterns of the isolates. J Nat Sc Biol Med. 2013;4:306-9.

Stoll BJ, Shane AL. Infections of Neonatal infant. In: Kliegman RM, Stanton BF, St Geme JW, Shor NF. Nelson textbook of Pediatrics. Elsevier Health; 2015:914-915.

Khante SV, Raunt SS. Clinical and bacteriological study of neonatal septicemia in a tertiary care hospital. Int J Res Med Sci. 2017;5:4455-62.

National Committee for clinical laboratory standards. Performance standards for antimicrobial disc susceptibility testing. Wayne PA:NCCLS; 2004 (Fourteenth informational supplement (M100-S14).

Ahmed ASMN, Chowdhury MAKA, Hoque M, Darmstad. Clinical and bacteriological profile of neonatal septicemia in a tertiary level pediatric hospital Bangladesh. Indian Pediatr. 2002;39:1034-39.

Shukla OS, Rawat A. Clinical profile and outcome of early onset neonatal sepsis in high risk very low birth weight neonates. Int J Contemp Pediatr. 2018;5:389-94.

Mathur M, Shah H, Dixit K, Khambadkone S, Chakrapani A, Irani S. Bacteriological profile of neonatal septicemia cases (for the year 1990-91). J Postgrad Med. 1994;40:18-20.

Rasul CH, Hassan MA, Habibullah M. Neonatal sepsis and use of antibiotic in a tertiary care hospital. Pak J Med Sci. 2007;23(1):78-81.

Iyer CR, Naveen G, Suma HR, Kumarguru BN, Swetha K, Janakiraman. Clinical profile and outcome of neonates with suspected sepsis from rural medical college hospital of south India. Int J Contemp Pediatr. 2018;5:55-60.

Jaswal RS, Kaushal RK, Goel A, Pathania K. Role of C-Reactive protein in deciding duration of antibiotic therapy in neonatal septicemia. Indian Pediatr. 2003;40:880-3.